Parts | Plasmid Backbones | BioBrick Prefix and Suffix | Standards | Assembly Standards | Assembly Methods
Plasmid Backbone Overview
- The Registry divides a plasmid into two separate components:
- the part
- the plasmid backbone
Why would you want to use more than one plasmid backbone?
One part can be used in several different plasmids backbones. It's easy to move a part from one plasmid backbone to another.
- Operate your part in the plasmid backbone that works for your system
- Maybe your part uses a lot of cellular resources, so you'll want to operate it in a low copy plasmid backbone
- Or you want to test your part in different organisms by using a multi-host plasmid backbone
- Use a plasmid backbone to measure your part
- You may want to use a plasmid backbone that will help you measure attributes of your part
- Have a library of promoters you want to test? Use a plasmid backbone with a built in coding region.
- Don't forget, documenting and characterizing your part is important!
- The Prefix and Suffix flank the part on either side:
- the part does not include the prefix and suffix
- the plasmid backbone is defined as the sequence starting with the suffix and ending at the prefix